Tissue microarray (TMA) slides containing primary breast cancers (ER+) and adjacent normal tissue specimens from 103 breast cancer cases were purchased from U.S. Biomax (Rockville, MD) (BR243u) and Alena Biotechnology Ltd. Co. (Xian, China) (BR1921c). Representative areas of each invasive carcinoma were identified on the corresponding hematoxylin and eosin (H&E)–stained slides. The TMA sections were immunohistochemically stained for PP2Cδ, p-BRCA1, and acetyl-p53 with a standard immunostaining protocol as previously described (41). The assessment of staining scores was described previously (42). In brief, the percentages of staining-positive cells were scored into four categories: 0 to 1 (0 to 25%), 1 to 2 (26 to 50%), 2 to 3 (51 to 75%), and 3 to 4 (76 to 100%). The staining intensities were scored into four grades: 0 (none), 1 (weak), 2 (moderate), 3 (strong), and 4 (very strong). The final staining score was defined as the product of the percentage and intensity scores. Correlations between tissue PP2Cδ level and p-BRCA1 or acetyl-p53 staining score were tested by Pearson’s rank correlation analysis, with R and P values indicated.

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