A p53-responsive reporter plasmid containing 13 copies of the p53-binding consensus sequence, pG13-LUC, was a gift from B. Vogelstein (Addgene plasmid no. 16442). Cells were transfected with pG13-LUC plasmid, and luciferase assays were carried out as previously described (40).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.