Recombinant p300 protein (1 μg) purified from baculovirus was preincubated with the indicated amounts of purified bacterially expressed BRCA1 protein or bovine serum albumin (BSA) for 10 min at room temperature. After preincubation, 1 μg of GST-p53 was added and p53 acetylation was measured as described previously (16).

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