To effectively target the BRCA1 gene for KO, CRISPR guide sequences were selected using the website based on the following criteria: The sgRNA sequence should have a high efficiency score and a high specificity score (low off-target effects). Sequences for sgRNA oligos can be found in table S1.

sgRNA oligos were cloned into vectors according to the provided protocols from Bauer et al.’s paper (39). The pSpCas9(BB)-2A-GFP plasmid, which contained both Cas9 endonuclease and enhanced GFP (EGFP) expression cassettes, was purchased from Addgene.

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