Immunoprecipitations, Western blot analysis, and immunoprecipitation Westerns were carried out by standard methods described before (18, 25). Densitometry was performed using Scion Image software (Scion Corp., Frederick, MD). Antibodies were obtained from commercial sources listed below. Anti-PP2Cδ, anti-ATM, anti-ATM (pS1981), anti–caspase-3, horseradish peroxidase (HRP)–anti-mouse immunoglobulin G (IgG), and HRP–anti-rabbit IgG were obtained from Santa Cruz Biotechnology; anti-p53, anti-p53 (acetyl K382), anti-p53 (acetyl K373), and anti-BRCA1 (pS1423) were provided by Abcam; and anti-BRCA1, anti-p300, anti–cleaved caspase-3, anti-actin, and anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were purchased from Cell Signaling Technology.

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