Human cell lines, including MCF-10A, MCF-7, H1299, and hTERT-HME1, were obtained from the American Type Culture Collection (Rockville, MD). The hTERT-HME1 cell line was cultured in MEBM supplemented with MEGM Mammary Epithelial Cell Growth Medium SingleQuots Supplements and Growth Factors. Cultures were maintained in a humidified incubator at 37°C with 5% CO2. These cells were authenticated by Laragen Inc. (Culver City, CA) by short tandem repeat profiling and monitoring cell morphology and biological behavior and tested to exclude mycoplasma contamination before experiments. For UV-induced damage, Stratalinker 1800 (Stratagene, La Jolla, CA) was used at 20 J/m2. Another agent used to induce DNA damage was Dox (Sigma-Aldrich) at 0.1 μM for MCF-10A and 0.5 or 1.0 μM for MCF-7. Standard cell culture and reverse transcription PCR were carried out as described previously (18, 36, 37).

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