Mouse peripheral blood was extracted after right atrial puncture into a 2-ml Eppendorf tube. Then, blood tubes were incubated at room temperature for coagulation (15 min). After incubation, tubes undergo a centrifugation step (450g), and serum was collected. For cytokine measurement, we used LEGENDplex Mouse Inflammation Kit (BioLegend) following the manufacturer’s instruction. Data were acquired on a CytoFLEX instrument (Beckman Coulter) and analyzed using LEGENDplex analysis software (BioLegend).

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