The cell viability was assessed using LIVE/DEAD staining kit (Molecular Probes, Life Technologies). The MBs were incubated for 30 min in PBS containing 1 μM calcein AM and 2 μM ethidium homodimer-1, in flushing 100 μl of the solution. The samples were then washed with PBS and imaged under a motorized fluorescence microscope (Nikon, France).

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