Wild-type N2 and isp-1(qm150) mutant animals were synchronized by using an embryo bleaching method (7), and synchronized larvae were fed with either control or vrk-1 RNAi bacteria until the worms reached young adult stage. Worms were harvested with M9 buffer, washed to remove remaining bacteria, and frozen in liquid nitrogen. Three independent biological repeats for each condition were used for mRNA-seq experiments. Total RNAs were isolated using RNAiso plus (Takara, Shiga, Japan), followed by ethanol precipitation. Paired-end mRNA-seq library was prepared and sequenced by Illumina platform at Macrogen (Macrogen Inc., Seoul, South Korea).

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