Synchronized L4 or young adult animals grown at 20°C were used for RNA extraction, cDNA synthesis, and qRT-PCR analysis. For qRT-PCR analysis of food-deprived animals shown in fig. S7A, day 3 adult worms were used. Briefly, RNAiso (Takara, Japan) was used to extract total RNA, and cDNA was obtained using ImProm-II Reverse Transcriptase (Promega, WI, USA). A 7300 Real-Time PCR System (Applied Biosystems) was used to perform quantitative PCR experiments, and the results were analyzed by using the comparative CT method described in the manufacturer’s manual. The average values of the mRNA levels of ama-1, tba-1, or pmp-3 gene were used for normalization. The average of at least two technical repeats was applied for each biological data point.

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