Analysis of muscle infiltration with FAPs was performed as previously explained (14). Briefly, pooled muscles (tibialis anterior, gastrocnemius, soleus, quadriceps, triceps, and pectoralis) were digested with collagenase type II and dispase. Mononuclear cells were stained with the following antibodies: phycoerythrin (PE)–Cy7–conjugated CD45 and CD31 (552848 and 561410, respectively; BD Biosciences), PE-conjugated PDGFRα (562776; BD Biosciences), and allophycocyanin (APC)–conjugated rat anti–integrin α7 (67-0010-05; Ablab), resuspended in PBS/1% FBS. Samples were run on a BD FACSAria instrument, and data were analyzed using FlowJo (BD Biosciences). All experiments were performed on at least three biological replicates.

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