For Western blot analyses, proteins were separated on 10% SDS–polyacrylamide gel electrophoresis gels and transferred to polyvinylidene difluoride membrane. Antibodies were diluted in 5% skim milk in TBST (tris-buffered saline with Tween 20) and incubated overnight at 4°C. Signal was visualized using Pierce ECL Western blotting substrate (Thermo Fisher Scientific).

Antibodies used in the study are as follows: rabbit anti-DUX4 (RD2-47c, 1:50, R&D Systems), GAPDH–horseradish peroxidase (HRP) (1:5000, 60004, Proteintech), mouse monoclonal acetylated histone H3 (D-4, 1:250, sc-518011, Santa Cruz Biotechnology), mouse monoclonal acetylated histone H4 (E-5, 1:250, sc-377520, Santa Cruz Biotechnology), mouse monoclonal histone H3 K9me3 (6F12-H4, 1:250, sc-130356, Santa Cruz Biotechnology), rabbit anti–histone H3 K18Ac [1:500, ab1191 (Abcam) and 9675T (Cell Signaling)], rabbit anti–histone H3 K27Ac (1:500, ab, Abcam), rabbit anti–acetylated lysine (1:500, 9441S, Cell Signaling), rabbit anti–histone H3 K9 (1:500, C5B11, Cell Signaling), anti–histone H3 (Poly6019, 1:500, 601901, BioLegend), and anti–rabbit-HRP (1:5000, 111-035-003, The Jackson Laboratory).

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