HMOs present in breast milk, infant stools, and culture supernatants were fluorescence-labeled and analyzed by HPLC using the method described previously (11). Maltoheptaose was added as an internal standard to breast milk and culture supernatant, while melibiose was used for fecal HMO analysis. Analysis of culture supernatants was carried out in technical triplicates, whereas breast milk and infant stool analyses were carried out once.

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