All animal experiments were approved by the Institutional Animal Care and Use Committee at the University of Chicago (protocol no. 72405) or the Research Institute at Nationwide Children’s Hospital (protocol no. AR17-00072) and adhered to the standards of the NIH Guide for the Care and Use of Laboratory Animals. For most experiments, female BALB/c and C57BL/6 mice were purchased from Taconic. For the MHC experiments, female BALB/c, C57BL/6, C.B10-H2b, and B6.C-H2d mice were purchased from Jackson. For the experiments using CB.17 mice, female CB.17, BALB/c, and C57BL/6 mice were purchased from Charles River Laboratories. 2W-OVA transgenic C57BL/6 mice were a gift from J.J. Moon (Harvard University) and were bred at the University of Chicago. Primary infection was performed when the mice were 7 to 8 weeks old.

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