For identification of deamidation sites, kRTA was purified from 293T cells transfected with a plasmid containing FLAG-kRTA by one-step affinity chromatography using anti-FLAG M2 agarose. Purified kRTA was subjected to SDS-PAGE and Coomassie staining. Gel slices containing RTA were prepared for in-gel digestion. MS analysis was performed by the Taplin Mass Spectrometry Facility of Harvard Medical School and Poochon Scientific (Frederick, MD).

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