qRT-PCR was performed as previously described (10). 293T, SLK, or HOK cells were harvested at various time points for total RNA extraction using TRIzol reagent (Invitrogen). cDNA was synthesized using a PrimeScript RT kit according to the manufacturer’s instruction (Invitrogen). cDNA was diluted 20-fold, and qRT-PCR was performed using SYBR Green Master Mix (Applied Biosystems) on the Applied Biosystems StepOnePlus Real-Time PCR System. Relative mRNA expression for each target gene was calculated by the 2−ΔΔCt method using β-actin as an internal control. Sequences of qRT-PCR primers are shown in Table 1.

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