iSLK/rKSHV.219, SLK/iBAC.RTA-WT, and SLK/iBAC.RTA-Q37 cells were reactivated with doxycycline (1 μg/ml) without or with 1 mM sodium butyrate for up to 96 hours as previously described (22). iSLK/RTA-WT or iSLK/RTA-DD cells infected with rKSHV.219 were induced with doxycycline (1 μg/ml) up to 96 hours. The supernatant containing infectious virion particles was collected and centrifuged at 1000g for 10 min. Virus was concentrated by ultracentrifugation at 32,500g for 2 hours, if necessary. Virus titer was quantified on 293T monolayers by flow cytometry with FACSCalibur (BD Biosciences, San Jose, CA). Data collected from fluorescence-activated cell sorting were analyzed with FlowJo software (TreeStar). Viral genomic DNA was purified from infected cells with the DNeasy Blood and Tissue Kit (Qiagen) for KSHV genome replication by quantitative RT-PCR (qRT-PCR)

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