iSLK/RTA-WT, iSLK/RTA-DD, SLK/iBAC.RTA-WT, or SLK/iBAC.RTA-Q37 cells were processed as previously described (53). Briefly, cells were fixed with 4% paraformaldehyde and permeabilized with 1% Triton X-100 buffer. After being blocked with goat serum, cells were incubated with primary mouse monoclonal anti-FLAG M2 antibody or polyclonal anti-RTA (1:100 dilution) serum. Cells were then incubated with Alexa Fluor 488–congugated goat secondary antibody (1:500 dilution), stained with 4′,6-diamidino-2-phenylindole (DAPI), and analyzed with confocal microscope (Nikon). Cells expressing fluorescent fusion proteins were fixed with 4% paraformaldehyde and directly analyzed by immunofluorescence microscope (Nikon). Representative images were shown for all analyses.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.