Aqueous GPC was used to characterize the molecular mass of PdBT, CS/PdBT, BMPm/PdBT, and NC/PdBT and compare their molecular mass distributions to those of the unbound biomolecules. Waters Systems (Milford, MA) components in the GPC system included a model 510 HPLC pump, model 410 differential refractometer, model 717 autosampler/injector, and an Ultrahydrogel (6 μm, 6 mm by 40 mm) analytical column. All samples and standards were dissolved at 10 mg/ml and run in 100 mM sodium nitrate at 0.5 ml/min, with filtration of all prepared solutions at 0.2 μm. Mn and PDI were calculated in Waters Empower software by comparison to standard curves (see fig. S4) generated using narrowly dispersed PEG standards of 450, 2500, 10,225, 30,250, 44,000, and 78,300 Da.

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