Cylindrical wells of prescribed dimensions (D = 6 mm, H = 1 cm) were generated by bonding PDMS-based walls to a glass slide through oxygen plasma treatment and were subsequently coated with collagen I. Hydrostatic pressure differentials ranging from 3 to 20 Pa were applied by adding (or removing in select experiments) 10- to 70-μl volumes of medium atop the cells seeded in the wells. For control (unstimulated) cases, no medium was added or removed.

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