The calcium assay was performed using the Fluo-4 Direct Calcium Assay Kit according to the manufacturer’s protocol (F10471, Invitrogen). Briefly, cells at ~80% confluency were incubated with a 0.4× reagent at 37°C and 5% CO2 for 45 min and then at room temperature for 15 min. Subsequently, cells were trypsinized and seeded into the devices for imaging. A 0.4× reagent was included in the medium during the experiments. Intracellular calcium imaging in transfected HEK293 cells was carried out in cells loaded with 4.5 μM Fura2-AM (Fura-2-acetoxymethyl ester) (Invitrogen) as previously described (28).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.