Cells were examined with an inverted Nikon Eclipse Ti microscope (Nikon, Tokyo, Japan) using a 40× air objective. Cell migration phenotype was tabulated manually. Blebbing cells were defined as those displaying membrane blebs, which were identified as discrete, spherical-like bulges localized at the cell poles. Mesenchymal cells were defined as those exhibiting finger-like protrusions.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.