Live cell videos were exported to ImageJ (National Institutes of Health, Bethesda, MD), and decision-making times were calculated manually. The decision-making time was defined as the time period from the first cell contact with the trifurcation t0 until complete nuclear entry to one of the branch channels t2 (t2 − t0). The decision-making time was rendered dimensionless by multiplying it with the cell speed at the feeder channel and dividing the product by the characteristic length scale of 10 μm.

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