Live cell videos were exported to ImageJ (National Institutes of Health, Bethesda, MD), and the protrusion length was measured manually. A protrusion, lp, was defined as the distance between leading edges of the cell and the nucleus provided that the nucleus had already emerged into the branch channel (Fig. 1D, iii). If not, lp simply corresponded to the protrusion length inside the branch (Fig. 1D, ii). The protrusion length lp was then normalized by the cell front-rear length, l0, inside the feeder channel (Fig. 1D, i).

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