PDMS-based microfluidic devices consisting of an array of trifurcating Ψ-like microchannels were fabricated as described previously (3, 10). All design dimensions used are summarized in table S1: The dimensions were verified using a laser profilometer. In migration assays, all channels were coated with collagen I (20 μg/ml; Collagen I Rat Protein, Tail, Thermo Fisher Scientific).

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