All results are expressed as the mean ± SEM or SD as indicated. One-way analysis of variance (ANOVA), Student’s t-test and Tukey’s post hoc tests were used when more than two groups were compared. Statistical differences in survival rate were determined with the log-rank test. All statistical analyses were performed using the Prism software package (Prism 5.0; GraphPad Software, USA, 2007). The threshold for statistical significance was P < 0.05.

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