Silver sand (VWR) and soil ( were used for ANR phenotyping experiments. Col-0 seeds were kept in 4°C for 3 days to ensure uniform germination and then sown in pots with sand or soil. The pots were kept under long-day photoperiod conditions (16-hour light/8-hour darkness, 22°C, and 60% humidity) for 8 days. Then, forceps were used to gently take the seedlings from the sand or soil. Seedlings were then washed using water before counting the ANRs under a microscope.

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