Candidate target genes’ coding sequence (CDS) was cloned in pcDNA3.1 (flag tag)/Puro. Control plasmid and the plasmid with the target genes’ CDS clones were transfected into the enhancer KO 22Rv1 cells. The cells were treated with puromycin to select the cells that stably expressed the target genes. qRT-PCR and Western blot were used to verify the stable expression of the target genes.

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