We first screened out overexpressing strains expressing the same recombinant protein by His tag antibody. The overnight cultured, overexpressing strains were diluted 1:1000 in LB medium or M9 medium and incubated at 37°C with 96-well plates, and each strain was added to six wells. The absorbance at 600 nm at initiation was set as a blank and then measured every hour using a spectrophotometer. All the growth dynamic curves were drawn using Origin 8.0.

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