For cell cycle analysis, T24 cells were plated at a density of 5 × 104 cells per well in 24-well plates. At 48 hours after transfection with HNTs/siRIPK4, HNTs/siNC, unloaded HNTs, or PBS, cells in each well were trypsinized, fixed with 70% ethanol, and then stained using a Coulter DNA-Prep Reagents Kit (Beckman Coulter, Fullerton, CA). Flow cytometry was then used to detect the DNA content in the cells of each sample (Becton Dickinson, San Jose, CA, USA).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.