Tumor cell line T24 (human bladder cells; American Type Culture Collection) was grown in Dulbecco’s modified Eagle’s medium (DMEM; CellGro) with 10% heat-inactivated fetal bovine serum (FBS) (CellGro) in 5% CO2 at 37°C. The oligoribonucleotide sequence of the siNC and human RIPK4 siRNA (siRIPK4) were as follows: 5′-GACACCAGCAAACUGAUGATT-3′ (sense) and 5′-UCAUCAGUUUGCUGGUGUCCC-3′ (antisense) (Shanghai GenePharma Co. Ltd., Shanghai, China). Fluorescein-tagged siRNA (FAM-siRNA) was synthesized using fluorescein modification of the 3′-end of the sense strand. Mouse antibodies recognizing RIPK4 and β-actin were obtained from Abcam (Cambridge, UK). Rabbit antibodies recognizing marker of proliferation Ki-67 (Ki-67), inhibitor of NF-κB (IκB) kinase subunit β (IKK), phospho (p)–IKK, IκB, p-IκB, and NF-κB p65 subunit (NF-κB-p65) antibodies were purchased from Cell Signaling Technology (Denver, MA, USA).

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