All procedures using animals were approved by the Salk Institute’s Institutional Animal Care and Use Committee. Eight-week-old female SCID/Beige (PrkdcscidLystbg-J/Crl; no. 250, Fox Chase) were intravenously injected with 1 × 106 luciferase-expressing A549 cells (n = 7 mice per condition). All mice were imaged via bioluminescence imaging (BLI) 20 min after intravenous injection. Mice were monitored weekly for lung tumor growth by BLI. For histopathology studies, lungs were inflated with 10% formalin, fixed in 10% formalin for 18 hours, and then paraffin-embedded.

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