ChIP was performed as described previously (44). For ChIP-seq experiments, libraries were prepared using NEBNext ChIP-seq reagents from Illumina. Samples were run on MiSeq using v2 chemistry with 25–base pair (bp) paired-end reads. FASTQ sequencing files were mapped to the genome using bowtie2, and peaks were identified. Differential binding and annotation to the genome were performed using HOMER.

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