Cells were transfected with the CRE-luciferase (pGL3 vector) or the ID1-Iuciferase reporter (pGL2 vector), and luciferase activity was measured in the GloMax-Multi Microplate Reader (Promega). Luciferase activity was normalized to β-galactosidase activity. When indicated, cells were treated with FSK. The ID1 promoter reporter construct was a gift from F. Ventura (34).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.