Specific antibodies against LKB1 (no. 3047, Cell Signaling Technology), tubulin (no. 05-829, Millipore), ID1 (BCH1/no. 195-14, BioCheck), HSP90 (sc-13119, Santa Cruz Biotechnology), and Lamin A/C (sc-7293, Santa Cruz Biotechnology) were used for immunoblot. For CREB, pCREB (pSer133), pCRTC2/3 (pS273) (43), and CRTC2 detection, rabbit polyclonal antibodies were raised against their respective antigens. FSK (Sigma-Aldrich) and the selective SIK inhibitor YKL-05-096 (a gift from N. S. Grey) were used at the indicated concentration. Nuclear and cytoplasmic fractions were isolated using an NE-PER nuclear and cytoplasmic extraction kit (Thermo Fisher Scientific) following the manufacturer’s conditions.

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