Fixed brain sections were incubated with DAPI for 15 min and mounted on glass slides (without immunostaining), and images of whole brain sections were taken using a slide scanner with a 20× objective (VS120, Olympus). For analysis, areas with GFP fluorescence in EC and CPu were outlined in each brain section and the mean ± SD fluorescence intensity of the areas was measured using ImageJ. For single-cell fluorescence, individual cell bodies were outlined manually in the EC and CPu injection sites, and their fluorescence intensity was measured.

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