The number of huTau donor cells, huTau recipient cells, Alz-50+ neurons (misfolding), and DAPI+ nuclei (to assess cell loss) in the EC, subiculum, and hippocampal formation of AAV-injected hemispheres was determined by counting all [GFP+], [huTau+/GFP], and [Alz-50+] cells in the area of interest, which was outlined as region of interest (ROI) using the CAST software (Olympus). The cell numbers were counted in three to four brain sections per mouse and three to five mice per group. For DAPI+ nuclei counting in the EC, we outlined layers 1, 2/3, and 4/5 of the EC and performed stereological counting of 20% of the nuclei in the ROI, followed by extrapolation to 100%. Counting was done on an Olympus BX51 light microscope equipped with a 20× objective.

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