Adult P. leucopus were obtained from the PGSC and maintained in the AAALAC (Association for Assessment and Accreditation of Laboratory Animal Care)–accredited University of California (UC) Irvine vivarium for 6 weeks before the experiment. Each animal was infected with an intraperitoneal injection of 50 μl of citrated blood freshly obtained from CB17 strain severe combined immunodeficiency mice (Charles River Laboratory). After 5 weeks, the animals were euthanized with carbon dioxide and terminal exsanguination, with blood and tissue samples collected. The protocol was approved by the Institutional Animal Care and Use Committee at UC Irvine and the Animal Care and Use Review Office of the United States Army Medical Research and Materiel Command.

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