The fraction of cells in G0 and G1 phases of the cell cycle was estimated by analyzing the results from Ki-67 staining and flow cytometry. First, the fraction of cells at the G0 phase was quantified as the fraction of cells that did not stain positive for Ki-67. Then, the fraction of cells at the G1 phase was determined by subtracting the estimated fraction of cells at the G0 phase from the fraction of cells determined to be at G0/G1 from flow cytometry (cells at G0/G1 from flow cytometry − cells at G0 from Ki-67 staining).

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