Human breast carcinoma specimens were obtained from the Stanford Tissue Bank. An International Review Board waiver was obtained for these studies, as the specimens were excess tissue not collected for the current research and were de-identified before use. Tissue samples were cut into 6-mm-diameter plugs and stored in serum-free RPMI 1640 at 4°C before testing. For mechanical testing, stress relaxation tests were performed using an Instron 5848 material testing system with a 4-mm indenter. The indenter was brought down to ~40% of strain, with a ramp of 0.5 mm mm−1 min−1 and held for 1 hour. The measurements were conducted with fully hydrated samples.

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