Animal studies to determine the role of ABCG2 in modulating PPIX distribution, metabolism, and excretion

WT and Abcg2-null mice were treated with D2-ALA (50 mg/kg, intraperitoneally), a stable isotope-labeled precursor of PPIX. One hour after D2-ALA treatment, liver and bile samples were collected for metabolomic analysis. In brief, liver and bile samples were analyzed using ultra-performance liquid chromatography coupled with a quadrupole time-of-flight mass spectrometer (UPLC-QTOFMS, Waters Corp, Milford, MA). Centroid and integrated mass chromatographic data were processed by MarkerLynx software (Waters Corp, Milford, MA) to generate a multivariate data matrix. These data were then exported to SIMCA-P+ software (Umetrics, Kinnelon, NJ) for multivariate data analysis. Principal components analysis and orthogonal projection to latent structures discriminant analysis were conducted to represent the major latent variables in the data matrix. The variables that substantially contributed to the discrimination between groups were subjected to structure identification.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.