WT, Abcg2-null, Fech-mut, and Fech-mut/Abcg2-null mice were used to determine the role of ABCG2 in EPP-associated phototoxicity. Phototoxicity was examined using a similar approach as described previously (33). In brief, the back skin of mice was shaved and exposed to ultraviolet (UV) light [DULEX, 395 to 410 nm, 294 lumens (lm)/m2] for 30 min each day and continued for 5 days. On the sixth day, all mice were euthanized. The back skin was harvested for histological analysis. Skin samples were also used for measurement of PPIX. In addition, red light (UltraFire, 630 nm, 6164 lm/m2) was used to determine whether the long-wavelength visible radiation can cause phototoxicity in Fech-mut and Fech-mut/Abcg2-null mice.

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