The aim of this study was to investigate the impact of confinement on cell cycle progression by studying cells cultured within confining viscoelastic hydrogels with different levels of stress relaxation in 3D matrices. The number of samples was determined on the basis of experimental approach, availability, and feasibility required to obtain definitive results. All samples were allocated randomly into experimental groups. No data were excluded from the analyses. The numbers of replicates are specified in the figure legends. The researchers were not blinded during data collection or analysis.

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