SA and CBD-SA were labeled with DyLight 800 NHS ester (Thermo Fisher Scientific) according to the manufacturer’s instructions. Unreacted dye was removed by a Zeba spin desalting column as described above. After labeling, 200 μg of each protein was injected intravenously into female FVB mice. Blood samples were collected in EDTA-coated tubes at 1 min, 1 hour, 4 hours, 24 hours, 74 hours, and 120 hours after injection. Blood samples were stored at 4°C until the end of sample collection. The samples were centrifuged (2000g, 5 min), and plasma was collected. Plasma samples were diluted in PBS and loaded into a 96-well black plate (100 μl/well). The concentration of each protein in plasma was measured with a LI-COR Infrared Odyssey Imager (Li-COR Biosciences). The method of curve fitting and calculation of plasma half-life was described above.

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