MMTV-PyMT cells or MC38 cells were seeded in a 96-well tissue culture plate (BD Falcon) at 3000 cells per well and incubated overnight. Cells were washed with DMEM [sodium pyruvate (110 mg/liter), 10% heat-inactivated FBS, 1% penicillin-streptomycin, and Phenol red (−)], and DMEM (80 μl/well) was added. Then, serial dilutions of aldoxorubicin, Dox-SA, or Dox-CBD-SA in PBS were added (20 μl/well). Cells were incubated 3 days at 37°C, and the viability was determined using CellTiter 96 AQueous One Solution Cell Proliferation Assay Kit (Promega) according to the manufacturer’s instructions. Cells treated with DMEM (80 μl/well) and PBS (20 μl/well) were defined as 100% viable, whereas the cell-free wells with the same mixture were defined as 0% viable. Half maximal inhibitory concentration (IC50) values were obtained by nonlinear regression analysis in Prism software ([inhibitor] versus normalized response).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.