SDS-PAGE was performed as described in our previous work (9). A 4 to 20% gradient gel (Bio-Rad) was used, and 0.5 μg of each Dox conjugate was loaded with or without reduction with 10 mM dithiothreitol. After electrophoresis, SimplyBlue SafeStain (Thermo Fisher Scientific) was used for gel staining according to the manufacturer’s instruction. ChemiDoc XRS+ system (Bio-Rad) was used for image acquisition.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.