Matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry

Purified CBD-SA was analyzed by MALDI-TOF MS (Bruker Ultraflextreme MALDI TOF/TOF) as described previously (9). Bruker flexControl was used for data acquisition, and Bruker flexAnalysis was used for data processing. First, a saturated solution of α-cyano-4-hydroxycinnamic acid (Sigma-Aldrich) was prepared in 50:50 acetonitrile:1% trifluoroacetic acid in water as a solvent. CBD-SA in phosphate-buffered saline (PBS; 5 μl, 0.1 mg/ml) and the matrix solution (25 μl) were mixed, and 1 μl of that mixture was dropped on the MTP 384 ground steel target plate. The drop was dried in a nitrogen gas flow. All samples were analyzed using high mass linear positive mode method with 2500 laser shots at a laser intensity of 75%. The measurements were externally calibrated at three points with a mix of carbonic anhydrase, phosphorylase B, and bovine SA (BSA).

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