MMTV-PyMT cells were obtained from spontaneously developed breast cancer in FVB-Tg (MMTV-PyMT) transgenic mice as described previously (9). The MC38 colon carcinoma cell line was provided by the R. Weichselbaum laboratory (University of Chicago). Dulbecco’s modified Eagle’s medium (DMEM; Gibco) supplemented with sodium pyruvate (110 mg/liter), 10% heat-inactivated fetal bovine serum (FBS), and 1% penicillin-streptomycin was used for both cell lines. The cell lines were checked for mycoplasma contamination by an IMPACT I pathogen test (IDEXX BioResearch).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.