MMTV-PyMT cells were obtained from spontaneously developed breast cancer in FVB-Tg (MMTV-PyMT) transgenic mice as described previously (9). The MC38 colon carcinoma cell line was provided by the R. Weichselbaum laboratory (University of Chicago). Dulbecco’s modified Eagle’s medium (DMEM; Gibco) supplemented with sodium pyruvate (110 mg/liter), 10% heat-inactivated fetal bovine serum (FBS), and 1% penicillin-streptomycin was used for both cell lines. The cell lines were checked for mycoplasma contamination by an IMPACT I pathogen test (IDEXX BioResearch).

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