On the basis of the pilot study results and literature, four to five mice per group were used for T cell response evaluation and six to seven mice per group were used for cancer immunotherapy study. All values shown in the manuscript were means ± SD. Comparisons between two groups or several groups were analyzed using unpaired Student’s t test or one-way analysis of variance (ANOVA), followed by Bonferroni post hoc test. *P < 0.05, **P < 0.01, and ***P < 0.001 were considered statistically significant. All statistical analyses were carried out using Prism 7.0 (GraphPad Software, La Jolla).

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