MAL-PEG3000-b-PLA2000 was purchased from JenKem Technology Co. Ltd. All DNA sequences, fluorophore-modified CpG sequences, and thiol-modified primer were synthesized in Integrated DNA Technologies. T4 DNA ligase (4000 U/μl), phi29 DNA polymerase (10 U/μl), deoxynucleotide triphosphate (dNTP) solution mix (10 mM each nt), and 10× bovine serum albumin (BSA) in buffer solution were all purchased from New England Biolabs, and Cy5-modified dUTP was purchased from Enzo Life Sciences. R848 was purchased from Sigma-Aldrich. aPD-1 antibody was purchased from Bio X Cell Inc. All the enzyme-linked immunosorbent assay (ELISA) kits used in this study were purchased from Thermo Fisher Scientific. Staining antibodies including anti–CD8-APC-Cy7, PD1-BV421, F4/80-PE (phycoerythrin), and CD11c-APC were purchased from BioLegend. Anti–CD80-PerCP-Cy5.5 and anti–CD86-PE were purchased from PeproTech. Anti-mouse SIINFEKL/H-2Kb-PE antibody was purchased from eBioscience. Dextramer-PE was purchased from Immudex. MC38 cell line was purchased from ATCC and cultured in Dulbecco’s modified Eagle’s medium, and DC2.4 cells were cultured in RPMI 1640 medium with 2 mM l-glutamine.

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